Phytochemical analysis the phytochemical analysis techniques were followed for solvent extracts of vitex negundo and datura metel using standard protocol 17. The qualitative uvvis spectrum profile of methanolic leaf extract of vitex negundo was selected from 250 nm to 900 nm due. Phytochemical studies on vitex negundo linn revealed the presence of volatile oil, triterpenes. The present study aimed to investigate the potential of salt stress to enhance the accumulation of the anticancer phytochemicals in andrographis paniculata accessions. In the present study the phytochemical compounds present in the ether extract of vitex negundo linn leaves extract analyzed and the free radical scavenging. Chloroform extract of catharanthus roseus shows highest antimicrobial. Phytochemical screening of vitex negundo species local. Recent days, ayurveda medicine is becoming one of the best alternatives for the modern medicines for effective control of cardiovascular disease cvd due to their limited side effects and availability to a common man. Phytochemical is a broad term meaning plant phyto chemical referring to a wide variety of compounds that occur naturally in plants. The extracts ethanol and distilled water were screened for the presence of. Phytochemical screening and antimicrobial activity of. General techniques involved in phytochemical analysis international journal of advanced research in chemical science ijarcs page 27 mass is packed and the top of the percolator is closed. Both the plants contained antioxidant phytochemicals such as alkaloids, tannin, saponins, phenolics and flavonoids.
Phytochemical analysis and antioxidant property of leaf. In the present study leaves of an important medicinal plant vitex negundo was subjected to a phytochemical screening, flavonoid content and characterization of flavonoids moiety of vitex negundo leaves extracts. International journal of research pharmaceutical and nano. Phytochemical analysis the preliminary phytochemical evaluation of leaves was carried on extract prepared by successive extraction method in soxhlet.
The presence of these phytochemicals can be correlated with medicinal potential of these plants. Chawla as, sharma ak, handa ss and dhar kl, chemical investigation and antiinflammatory activity of vitex negundo seeds, j nat prod, 1992, 552, 163167. Plants are used in traditional medicine contain a vast array of substances that can be used to treat chronic and even infectious diseases. Zahoor ullah 1,riaz ullah 1, 2, azharulhaq ali shah 2, ijaz ahmad 2 and sajjad haider 1 department of chemical engineering, college of engineering, king saud university 1, p. Phytochemical and antibacterial screening on leaf extracts.
Agarwood is a highly valuable fragrant resinous wood and has been listed as endangered species in recent years. International journal of research in pharmaceutical and nano sciences. For this purpose, 70dayold plants were grown in different salinity levels 0. Qualitative phytochemical analysis of these plants confirms the presence of various phytochemicals like alkaloids, flavonoids, steroids and terpenoid. Preliminary phytochemical analysis of leaf powder extracts. Phytochemical constituents and pharmacological activities. The previously dried powdered leaves 50 gm were extracted in a soxhlet apparatus with ethanol and water successively. Phytochemical analysis shows the presence of flavonoids, tannins triterpenoids, saponins, sterols, alkaloids and carbohydrates.
Plants have a great potential for producing new drugs for human benefits. Molecular docking analysis was carried out by molegro virtual docker software, to screen the 16 chosen compounds and rank them according to their binding affinity towards the site of interaction of the. Preliminary phytochemical analysis and thin layer chromatography of two strongly active plant extracts namely roots of vitex negundo l. Ms method was established to rapidly identify authenticity of agarwood without sample preparation, some 2. Preliminary phytochemical analysis of the extracts of. Phytochemical analysis and evaluation of antioxidant activity of. Antimicrofilarial activity of methanolic extract of vitex. The phytochemical screening of the two plants showed presence of alkaloids, carbohydrates, glycosides, phenolic and tannin compounds, flavonoids, saponins.
Phytochemical and pharmacognostic analysis and thin layer chromatography were carried out on, vitex negundo l. Evaluation of phytochemical and antimicrobial study of extracts of. Vitex negundo nirgundi is one of the widely studied and used medicinal plants amongst those mentioned in ancient books. It was possible to identify 24 alkaloids from a pool of 60 signals.
Pdf phytochemical screening of vitex negundo praveen. Screening of antioxidant property and phytochemical constituents. Essential oils of azadirachta indica and vitex negundo. The alkaloid patterns in wild bulbs, in vitro bulbs and callus obtained by micropropagation of rhodophiala pratensis were investigated using gas chromatographymass spectrometry. Materials and methods plant material vitex negundo l. A total of 16 compounds present in vitex negundo, based on a comprehensive literature survey was selected for this molecular screening. Leaves were also carried out to detect the major phytochemical groups present in these plants. Analysis of the anticancer phytochemicals in andrographis. Qualitative phytochemical screening phytochemical analysis of each extract has been carriedout according to standard protocols anyasor et al. The goals of the study were to determine and compare the phytochemical screening, antioxidant and antimicrobial activities of the essential oils of azadirachta indica and vitex negundo.
Phytochemical constituents phytochemical studies on vitex negundo linn revealed the presence of volatile oil, triterpenes, diterpenes, sesquiterpenes, lignan, flavonoids, flavones, glycosides, iridoid glycosides and stilbene derivative. General techniques involved in phytochemical analysis. Herbal medicine, rather than merely herbal medicine, rather than merely curing a particular disease, aims at returning the body back to its natural state of health. Pharmacognostic and phytochemical analysis of vitex negundo. Phytochemical studies includes determination of water extractive value of the leaves of vitex negundo, qualitative analysis of the water extract and thin layer chromatographic studies of water extract with. Phytochemical diversity in essential oil of vitex negundo. Senthikumara nadar college, virudhunagar, tamilnadu, india abstract. Nair department of environmental sciences, all saints college, department of biotechnology, university of kerala, kariavattom campus 695 581 email. The phytochemical analysis carried out on the plant stembark extract revealed the presence of alkaloids, saponins, tannins, glycosides and flavonoids.
The plant species above described were used for treating disease in natural therapeutics and in the form of chemical compounds but it was. The phytochemical screening and antioxidant property of fresh leaves of vitex doniana and mucuna pruriens, used in the management and treatment of various diseases, were studied. Research article phytochemical analysis and antibacterial. Adhatoda vasica and vitex negundo leaf sample with abstract the leaf samples of adhatoda vasica and vitex negundo were subjected to phytochemical analysis. Phytochemical and pharmacological profile of vitex negundo. The name chinese chaste tree is derived from one of its therapeutic activity, which depresses the sexual desire. Wadood a, ghufran m, jamal sb, naeem m, khan a, et al. Phytochemical analysis phytochemical analysis was carried out in the methenolic extract of the leaves of vitex negundo linn using standard procedures to identify constituents as described by harbone1984,trease and evans 1979 and sofowara 1993.
International journal of health sciences and research. Materials and method plant material the leaves of vitex negundo were collected in the local sivakasi from lands. The analysis showed alternation in 29 the alkaloids type ratio and number of compounds between wild bulbs, in vitro bulbs and callus. The leaf powder of the study plant was dissolved in various solvents and the preliminary phytochemical tests were carried out 3. The petroleum ether, chloroform, ethanol, methanol and aqueous extracts were prepared, and further screened for preliminary phytochemical test. The detailed of phytochemical constituents is present in each part of the plant is given below. January february 45 pathogenic microbes has further necessitated the need to search for newer antibiotic sources. Save nature to survive phytochemical screening and. Request pdf phytochemical and biological evaluation of vitex negundo linn.
Alkaloids were detected in extracts of vitex doniana only, while cardiac glycosides were also present in extracts of mucuna pruriens only. Table 1 shows the results of phytochemical analysis of the four extracts. Salinity causes the adverse effects in all physiological processes of plants. The aim of the present study is phytochemical and pharmacological activities of a medicinally well known plant vitex negundo.
Petroleum ether extract of vitex negundo contains steroids, flavonoids, tannin and sugars are present. Phytochemical screening and antimicrobial activity of vitex. The phytochemical screening revealed the presence of phlobatannins, carbohydrates, tannins, glycosides, volatile oils, resins, balsams, flavonoids and saponins, while terpenes, sterols, anthorquinones and phenols were absent. Anticancer screening of the phytochemicals present in the.
Pharmacological and phytochemical evidences for the. Phytochemical constituents and pharmacological activities of vitex. Phytochemical analysis phytochemical analysis of extract for qualitative detection of alkaloids, flavonoids, steroid,volatile oil, glycoside, reducing sugar, tannins and saponins was performed by the extracts. Pdf phytochemical screening of vitex negundo praveen kumar. To analyse elemental profile, evaluate functional groups and find out phytochemical constituents of vitex negundo l.
Pdf preliminary phytochemical and biochemical studies on. The predominant components present in all the extracts of vitex negundo are flavonoids, terpenoids and steroids. The long list of thesaurus is result of its wide distribution throughout the world. The leaves powder was successively extracted with petroleum ether, chloroform, ethanol, water, hydroalcoholic. Molecular docking studies of phytochemicals of vitex.
Its important constituents are vitexin, nishindaside and. The stembark of the plant was extracted and subjected to phytochemical and antibacterial screening. To explore the diversity of the essential oil yield and composition of v. Chopra rn, nayar sl and chopra ic, glossary of indian medicinal plants publications and. Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. The phytochemical screening revealed the presence of. Pdf the biological investigations of vitex negundo l verbenaceae methanol extract of leaves were screened for antibacterial, minimal. Paikara et al phytochemical analysis and antimicrobial activity of indian j.
Vitex doniana were analyzed to substantiate its use in the ethnomedicinal practises. Phytochemical analysis of medicinal plants occurring in. Saponins, tannins, anthraquinones, terpenoids, and flavonoids were found in all the extracts. Medicinal uses and biological activities of vitex negundo. Phytochemical analysis of eight medicinal plants from. Phytochemical and antimicrobial screening of the stembark. Extracts abollfazl shakeri1, nourallah hazeri1, jafar vlizadeh2, ali ghasemi2, and fatemeh zaker tavallaei3 1departement of chemistry, university of sistan and baluchestan, p. Lamiaceae is a medicinal shrub which is known to exhibit curative activity against several ailments like relieve catarrh.
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